Patients older than 60 years had a significantly lower TCR repertoire diversity compared to younger (p = 0.03, Wilcoxon rank sum test). By using the MiXCR and Immunarch bioinformatic pipelines we analyzed the TCR repertoire diversity, as measured by the Chao1 estimation, and used the median value in the cohort as a cut-off for high and low TCR repertoire diversity, respectively.Īs of March 2021, analysis of 92 patients had been finalized. All patients were included in a nationwide Danish cohort with full long-term follow-up data on survival, infections, transfusion history and secondary cancers. In 288 patients with aggressive lymphoma (predominantly B-cell NHL), we have sequenced the circulating TCR repertoire in the autologous harvest products used in the ASCT. We performed high-throughput RNA based sequencing of the V, D and J segment of TCR beta chain with the Illumina Immune Repertoire NGS panel.
We explored how the systemic T-cell diversity impacted the long-term outcomes in lymphoma patients undergoing high-dose chemotherapy and autologous stem cell transplantation (ASCT). Poorer diversity of the TCR repertoire has been associated with inferior outcomes in other cancers. Novel NGS sequencing has made it possible to identify hundred thousands of T-cell clones in a single sample, consequently termed the TCR repertoire. The T-cell receptor (TCR) is exclusive for every T-cell clone and thus provides unique information about the condition of the immune system. The adaptive immune response, and especially the role of T-cells has become a key focus in the treatment of lymphoma. of Clinical Immunology, Copenhagen, Denmark, 8 Aarhus University Hospital, Hematology, Aarhus, Denmark, 9 Aalborg University Hospital, Aalborg, Aalborg, Denmark, 10 Odense University Hospital, Hematology, Odense, Denmark of Clinical Immunology, Copenhagen, Denmark, 4 Aarhus University Hospitalet, Clinical Immunology, Aarhus, Denmark, 5 Aarhus University Hospital, Clinical Immunology, Aarhus, Denmark, 6 Aalborg University Hospital, Clinical Immunology, Aalborg, Denmark, 7 Odense University Hospital, Dept. Husby 1ġ Rigshospitalet, Hematology, Copenhagen N, Denmark, 2 Finsen laboratory, Hematology-Oncology, Rigshospitalet, Copenhagen, Denmark, 3 Rigshospitalet, Dept.
LYMPHOMA BIOLOGY 089 | CLINICAL IMPACT OF T-CELL RECEPTOR REPERTOIRE DIVERSITY IN PATIENTS WITH LYMPHOMA UNDERGOING AUTOLOGOUS STEM CELL TRANSPLANTATION
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